Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

A new approach for simultaneous screening and quantification of toxic pyrrolizidine alkaloids in some potential pyrrolizidine alkaloid-containing plants by using ultra performance liquid chromatography–tandem quadrupole mass spectrometry

Language

English

Abstract

A rapid, but sensitive and selective method for simultaneous screening and quantification of toxic pyrrolizidine alkaloids (PAs) by ultra performance liquid-chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) on a tandem quadrupole mass spectrometer (TQ-MS) is described. This was accomplished by incorporating the precursor ion scan (PIS) acquisition and multiple reaction monitoring (MRM) acquisition in the same UPLC-MS/MS run. Notably, the developed PIS approach for detecting two pairs of characteristic product ions at m/. z 120/138 or 168/150, allowed specific identification of toxic retronecine and otonecine types PAs. This PIS method is highly sensitive with over 10-fold sensitivity improvement upon previously published LC-MS method. Moreover, this new approach is suitable for high sample throughput and was applied to the screening and quantifying toxic PAs in 22 samples collected from seven. Parasenecio species and four Senecio species. In addition, coupling the MRM with PIS approach generated quantitative results equivalent to those obtained by conventional MRM-only approach. This coupled MRM with PIS approach could provide both qualitative and quantitative results without the need of repetitive analyses. © 2010 Elsevier B.V.

Keywords

Multiple reaction monitoring (MRM), Parasenecio species, Precursor ion scan (PIS), Pyrrolizidine alkaloids (PAs), Senecio species, UPLC-TQ-MS

Publication Date

2010

Source Publication Title

Analytica Chimica Acta

Volume

681

Issue

2-1

Start Page

33

End Page

40

Publisher

Elsevier Masson

DOI

10.1016/j.aca.2010.09.011

Link to Publisher's Edition

http://dx.doi.org/10.1016/j.aca.2010.09.011

ISSN (print)

00032670

ISSN (electronic)

18734324

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