Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Amperometric bienzyme glucose biosensor based on carbon nanotube modified electrode with electropolymerized poly(toluidine blue O) film

Language

English

Abstract

The amperometric bienzyme glucose biosensor utilizing horseradish peroxidase (HRP) and glucose oxidase (GOx) immobilized in poly(toluidine blue O) (PTBO) film was constructed on multi-walled carbon nanotube (MWNT) modified glassy carbon electrode. The HRP layer could be used to analyze hydrogen peroxide with toluidine blue O (TBO) mediators, while the bienzyme system (HRP + GOx) could be utilized for glucose determination. Glucose underwent biocatalytic oxidation by GOx in the presence of oxygen to yield H 2O2 which was further reduced by HRP at the MWNT-modified electrode with TBO mediators. In the absence of oxygen, glucose oxidation proceeded with electron transfer between GOx and the electrode mediated by TBO moieties without H2O2 production. The bienzyme electrode offered high sensitivity for amperometric determination of glucose at low potential, displaying Michaelis-Menten kinetics. The bienzyme glucose biosensor displayed linear response from 0.1 to 1.2 mM with a sensitivity of 113 mA M -1 cm-2 at an applied potential of -0.10 V in air-saturated electrolytes. © 2010 Elsevier Ltd.

Keywords

Bienzyme glucose biosensor, Carbon nanotubes, Poly(toluidine blue O), Reaction mechanism

Publication Date

2010

Source Publication Title

Electrochimica Acta

Volume

55

Issue

23

Start Page

7055

End Page

7060

Publisher

Elsevier

DOI

10.1016/j.electacta.2010.06.074

ISSN (print)

00134686

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