Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Analysis of urinary porphyrins by high performance liquid chromatography-electrospray ionization mass spectrometry

Language

English

Abstract

A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra- and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0nM, respectively. CP was detected as the predominant species at 135.6nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases. Copyright © Taylor & Francis Group, LLC.

Keywords

electrospray ionization, high performance liquid chromatography, human urine, mass spectrometry, porphyrins, solid-phase extraction

Publication Date

2011

Source Publication Title

Journal of Liquid Chromatography and Related Technologies

Volume

34

Issue

15

Start Page

1578

End Page

1593

Publisher

Taylor & Francis

DOI

10.1080/10826076.2011.575981

ISSN (print)

10826076

ISSN (electronic)

1520572X

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