http://dx.doi.org/10.1002/elps.201000453">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Determination of stimulants and narcotics as well as their in vitro metabolites by online CE-ESI-MS

Language

English

Abstract

A simple, rapid and sensitive CE-ESI-MS method for the simultaneous analysis of seven stimulants and narcotics (amphetamine, ephedrine, methadone, pethidine, tetracaine, codeine and heroin) was developed. The CE-ESI-MS experimental conditions were optimized as follows: 20mmol/L ammonium acetate with pH 9.0 as running buffer, the separation voltage of 22kV and the sheath liquid of isopropanol/water (1:1 v/v) containing 7.5mmol/L acetic acid with 3.0μL/min flow rate. Under the optimized conditions, the stimulants and narcotics were well separated within 4.6min using a 70-cm length fused-silica capillary (50μm id). The detection limits (S/N=3) of the CE-ESI-MS analysis were in the range of 0.40-1.0ng/mL. Method repeatability of intra-day and inter-day was satisfactory. The recoveries obtained from the analysis of spiked urine samples were between 84.1 and 108%. The developed method was successfully applied for the simultaneous analysis of methadone, pethidine and codeine and their in vitro metabolites. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

CE-MS, Metabolites, Narcotics, Stimulants

Publication Date

2011

Source Publication Title

ELECTROPHORESIS

Volume

32

Issue

4-3

Start Page

472

End Page

478

Publisher

Wiley-VCH Verlag

ISSN (print)

01730835

ISSN (electronic)

15222683

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