Department of Chemistry
Determination of stimulants and narcotics as well as their in vitro metabolites by online CE-ESI-MS
A simple, rapid and sensitive CE-ESI-MS method for the simultaneous analysis of seven stimulants and narcotics (amphetamine, ephedrine, methadone, pethidine, tetracaine, codeine and heroin) was developed. The CE-ESI-MS experimental conditions were optimized as follows: 20mmol/L ammonium acetate with pH 9.0 as running buffer, the separation voltage of 22kV and the sheath liquid of isopropanol/water (1:1 v/v) containing 7.5mmol/L acetic acid with 3.0μL/min flow rate. Under the optimized conditions, the stimulants and narcotics were well separated within 4.6min using a 70-cm length fused-silica capillary (50μm id). The detection limits (S/N=3) of the CE-ESI-MS analysis were in the range of 0.40-1.0ng/mL. Method repeatability of intra-day and inter-day was satisfactory. The recoveries obtained from the analysis of spiked urine samples were between 84.1 and 108%. The developed method was successfully applied for the simultaneous analysis of methadone, pethidine and codeine and their in vitro metabolites. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
CE-MS, Metabolites, Narcotics, Stimulants
Source Publication Title
Lu, Minghua, Qin Li, Yongquan Lai, Lan Zhang, Bin Qiu, Guonan Chen, and Zongwei Cai. "Determination of stimulants and narcotics as well as their in vitro metabolites by online CE-ESI-MS." ELECTROPHORESIS 32.4-3 (2011): 472-478.