Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

GC/MS-based metabolomics reveals fatty acid biosynthesis and cholesterol metabolism in cell lines infected with influenza A virus

Language

English

Abstract

Metabolomics is the downstream of systems biology and has drawn significant interest for studying the metabolic networks from cells to organisms. To profile the metabolites in two different cell lines (A549 and AGS) infected with influenza A virus, gas chromatography coupled with mass spectrometry (GC/MS) was employed. Some differentiating metabolites in the cell lines were tentatively identified using reference library, interpreted and visualized by applying principal components analysis (PCA) and cluster heat map. Consequently, metabolic flux profiling allowed the differentiation of fatty acid biosynthesis and cholesterol metabolism during viral replication in the cell lines. The change in fatty acid turnover was also observed. Metabolomics investigation also revealed the different responses between A549 and AGS cell lines to the virus infection. From the pattern recognition results, AGS cell line might be more susceptible to influenza A virus. Regarding the fact that AGS is a poorly differentiated gastric adenocarcinoma cell line whereas A549 is a relatively differentiated lung tumor one, it is speculated that viral replication might be associated with the cell differentiations. © 2010 Elsevier B.V.

Keywords

GC/MS, Influenza A virus, Metabolomics, Multivariate data analysis

Publication Date

2010

Source Publication Title

Talanta

Volume

83

Issue

1

Start Page

262

End Page

268

Publisher

Elsevier

DOI

10.1016/j.talanta.2010.09.019

ISSN (print)

00399140

ISSN (electronic)

18733573

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