Department of Chemistry
Glucose biosensor based on nanohybrid material of gold nanoparticles and glucose oxidase on a bioplatform
A simple and relatively cheap glucose biosensor based on a combination of gold nanoparticles (Au NPs) and glucose oxidase (GOx) immobilized on a bioplatform eggshell membrane was established. Scanning electron microscopy showed successful immobilization of Au NPs/GOx on the eggshell membrane. The effects of pH, phosphate buffer concentration, and temperature on the glucose biosensor were studied in detail. The biosensor shows a linear response at a glucose concentration range of 5-525 μM. The detection limit of the biosensor is 2.5 μM (S/N = 3). The biosensor exhibits good repeatability with RSD = 3.6% (n = 6), good operational stability with over 300 measurements and long-term storage stability with a shelf life of at least 6 months. The response time is less than 60 s. The glucose level in commercial food samples has been successfully determined. The proposed work shows potential to develop cost-effective biosensors for biotechnological, biomedical and industrial use. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Biosensors, Eggshell membrane, Enzymes, Glucose oxidase, Gold nanoparticles
Source Publication Title
Zhang, Yan, Wenjuan Jia, Miao Cui, Chuan Dong, Shaomin Shuang, Yuen Kwan, and Martin M. F. Choi. "Glucose biosensor based on nanohybrid material of gold nanoparticles and glucose oxidase on a bioplatform." Biotechnology Journal 6.5 (2011): 492-500.