Department of Chemistry
Glucose biosensor based on nanohybrid material of gold nanoparticles and glucose oxidase on a bioplatform
A simple and relatively cheap glucose biosensor based on a combination of gold nanoparticles (Au NPs) and glucose oxidase (GOx) immobilized on a bioplatform eggshell membrane was established. Scanning electron microscopy showed successful immobilization of Au NPs/GOx on the eggshell membrane. The effects of pH, phosphate buffer concentration, and temperature on the glucose biosensor were studied in detail. The biosensor shows a linear response at a glucose concentration range of 5-525 μM. The detection limit of the biosensor is 2.5 μM (S/N = 3). The biosensor exhibits good repeatability with RSD = 3.6% (n = 6), good operational stability with over 300 measurements and long-term storage stability with a shelf life of at least 6 months. The response time is less than 60 s. The glucose level in commercial food samples has been successfully determined. The proposed work shows potential to develop cost-effective biosensors for biotechnological, biomedical and industrial use. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Biosensors, Eggshell membrane, Enzymes, Glucose oxidase, Gold nanoparticles
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Zhang, Yan, Wenjuan Jia, Miao Cui, Chuan Dong, Shaomin Shuang, Yuen Kwan, and Martin M. F. Choi. "Glucose biosensor based on nanohybrid material of gold nanoparticles and glucose oxidase on a bioplatform." Biotechnology Journal 6.5 (2011): 492-500.