http://dx.doi.org/10.1016/j.snb.2010.09.051">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Gold nanoparticles-coated eggshell membrane with immobilized glucose oxidase for fabrication of glucose biosensor

Language

English

Abstract

This work reports the fabrication and application of a glucose biosensor based on the catalytic effect of gold nanoparticles (AuNPs) on enzymatic reaction for blood glucose determination. AuNPs were initially in situ synthesized on the surface of an eggshell membrane (ESM) which was subsequently immobilized with glucose oxidase (GO x) to produce a GO x-AuNPs/ESM. The GO x-AuNPs/ESM was positioned on the surface of an oxygen electrode to form a GO x-AuNPs/ESM glucose biosensor. The effects of pH, concentration of phosphate buffer solution and amount of GO x on the response of the GO x-AuNPs/ESM glucose biosensor were studied in detail. AuNPs on GO x/ESM can improve the calibration sensitivity (30% higher than GO x/ESM without AuNPs), stability (87.3% of its initial response to glucose after 10-week storage) and shortens the response time (<30 s) of the glucose biosensor. The linear working range for the GO x-AuNPs/ESM glucose biosensor is 8.33 μM to 0.966 mM glucose with a detection limit of 3.50 μM (S/N = 3). The biosensor has been successfully applied to determine the glucose in human blood serum samples and the results compared well to a standard spectrophotometric method commonly used in hospitals. Our work demonstrates that the developed GO x-AuNPs/ESM glucose biosensor has potential in biomedical analysis. © 2010 Elsevier B.V. All rights reserved.

Keywords

Eggshell membrane, Glucose biosensor, Glucose oxidase, Gold nanoparticles

Publication Date

2011

Source Publication Title

Sensors and Actuators B: Chemical

Volume

152

Issue

1

Start Page

49

End Page

55

Publisher

Elsevier

ISSN (print)

09254005

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