http://dx.doi.org/10.1016/j.talanta.2011.05.007">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Hippocampal metabolomics reveals 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity associated with ageing in Sprague-Dawley rats

Language

English

Abstract

Metabolomics, the exponentially developing technique, could provide a systemic mapping in toxicology by directly measuring small molecular metabolites. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) was found to be neurotoxic in mammalian animals. In this study, we employed liquid chromatography/quadrupole time-of-flight mass spectrometry for non-targeted analysis of metabolic profiling in hippocampal sample sets of the rats exposed to TCDD. Hippocampal metabolome from different ages of the healthy rats (4-week, 12-week and 20-week) was also deciphered. The relationship between the two tested cases was unlocked to delineate TCDD toxicity associated with ageing. Tandem mass spectrometry fragmentation in conjunction with metabolic database searching and compared to authentic standards was utilized for metabolite identification. As a consequence, the reduced levels of phenylalanine and leucine/isoleucine as well as the up-regulation of inosine and hypoxanthine were highlighted for understanding of TCDD toxicity related to age in rats and the trajectory was depicted by principal components analysis. © 2011 Elsevier B.V.

Keywords

Ageing, Hippocampus, LC/MS, Metabolome, TCDD toxicity

Publication Date

2011

Source Publication Title

Talanta

Volume

85

Issue

2

Start Page

1007

End Page

1012

Publisher

Elsevier

ISSN (print)

00399140

ISSN (electronic)

18733573

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