http://dx.doi.org/10.1007/s00216-010-4463-4">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Proteomics investigation on aristolochic acid nephropathy: A case study on rat kidney tissues

Language

English

Abstract

Prolonged intake of aristolochic acid (AA) has been shown to be associated with the development of certain renal disorders. Renal tubular atrophy and interstitial fibrosis are the early symptoms of AA nephropathy. The symptoms were observed in rats that were dosed with AA at a dosage of 10 mg/kg/day for 1 month. Apart from the renal tubular atrophy and interstitial fibrosis, AA-DNA adducts were detected in the rat kidney tissue. Differentiated proteins were identified in the kidney tissues from proteomics investigations. The upregulated proteins identified included ornithine aminotransferase, sorbitol dehydrogenase, actin, aspartoacylase, 3-hydroxyisobutyrate dehydrogenase, and peroxiredoxin-1. Downregulated proteins such as ATP synthase subunit β, glutamate dehydrogenase 1, regucalcin, glutamate-cysteine ligase regulatory subunit, dihydropteridine reductase, hydroxyacyl-coenzyme A dehydrogenase, voltage-dependent anion-selective channel protein 1, prohibitin, and adenylate kinase isoenzyme 4 were also identified. Several identified protein markers were found to have biological and medical significance. © 2010 Springer-Verlag.

Keywords

Aristolochic acid, Kidney disease, Protein biomarker, Proteomics

Publication Date

2011

Source Publication Title

Analytical and Bioanalytical Chemistry

Volume

399

Issue

10

Start Page

3431

End Page

3439

Publisher

Springer Verlag

ISSN (print)

16182642

ISSN (electronic)

16182650

This document is currently not available here.

Share

COinS