http://dx.doi.org/10.1021/ac1025896">
 

Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' → 5' exonuclease activity

Language

English

Abstract

A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1. © 2010 American Chemical Society.

Publication Date

2011

Source Publication Title

Analytical Chemistry

Volume

83

Issue

2

Start Page

463

End Page

466

Publisher

American Chemical Society

ISSN (print)

00032700

ISSN (electronic)

15206882

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