Department of Chemistry
Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' → 5' exonuclease activity
A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1. © 2010 American Chemical Society.
Source Publication Title
American Chemical Society
Leung, Chung-Hang, Daniel Shiu-Hin Chan, Bradley Yat-Wah Man, Chuan-Jen Wang, Wing Lam, Yung-Chi Cheng, Wang-Fun Fong, Wen-Luan Wendy Hsiao, and Dik-Lung Ma. "Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3' → 5' exonuclease activity." Analytical Chemistry 83.2 (2011): 463-466.