Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Simultaneous synthesis and functionalization of water-soluble up-conversion nanoparticles for in-vitrocell and nude mouse imaging

Language

English

Abstract

Water-solubility and biocompatibility are prerequisites for rare-earth up-converting nanophosphors applied to biological imaging. In this work, we have developed a facile and one-step synthesis technique, through which water-soluble NaYF4: Yb3+, Er3+ nanoparticles (NPs) with functional groups including 3-mercaptopropionic acid, 6-aminocaproic acid and poly(ethylene glycol)methyl ether on their surface can be directly prepared without any further surface treatment. Some inorganic salts will be selected as starting materials, water and some low toxic organic agents have been used as reaction media, which differs from earlier works. Structural and up-converting fluorescence are characterized by a variety of techniques. Cell uptake and in-vitro imaging of the as-synthesized NPs have been investigated using a multiphoton con-focal laser scanning microscope with a near-infrared excitation source. Internalization of the bare and functionalized NPs in human lung carcinoma A549 and human cervical carcinoma HeLa cells are studied at a nanoparticle loading of 10 g mL-1 over an exposure period from 30 min to 24 h. The cytotoxicity of modified NPs in HeLa cells is found to be low. In addition, the feasibility of the NPs in animal imaging has been demonstrated by subcutaneously injecting these NPs into nude mouse. The results indicated that our directly synthesized NPs coated with various functional groups are promising as bio-imaging agents due to their easy uptake, long lasting, low cytotoxicity, emissive in various human carcinoma cell lines and small animals through up-conversion with near-infrared excitation. © 2011 The Royal Society of Chemistry.

Publication Date

2011

Source Publication Title

Nanoscale

Volume

3

Issue

5

Start Page

2175

End Page

2181

Publisher

Royal Society of Chemistry

DOI

10.1039/C1NR10090D

Link to Publisher's Edition

http://dx.doi.org/10.1039/C1NR10090D

ISSN (print)

20403364

ISSN (electronic)

20403372

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