Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Determination of puerarin in pharmaceutical and biological samples by capillary zone electrophoresis with UV detection

Language

English

Abstract

A rapid and efficient capillary zone electrophoresis (CZE) method with ultraviolet detection has been successfully developed for the determination of puerarin in Xinkeshu capsules and biological samples. The optimal CZE conditions were determined to be 70 mM H 3BO 3-Na 2B 4O 7, pH 9.2, injection time 5 s, applied voltage 25 kV, working temperature 25 °C and detection wavelength 254 nm. Under these conditions, a linear range from 17.3 to 138 μg/mL with the correlation coefficient of 0.9998 and limit of detection of 34.6 ng/mL (S/N = 3) for puerarin were obtained. The relative standard deviation of the migration time and peak area of puerarin were 0.23-0.91, 0.43-1.62, 0.10-0.64 and 0.64-1.32% for intra-day and inter-day analyses, respectively. Satisfactory results were obtained for recovery and repeatability. The proposed method was successfully applied to analyze the puerarin content in Xinkeshu capsules and some biological samples with good results. It is suitable for applications in pharmaceutical industries for quality control and in clinical laboratories for therapeutic drug monitoring purposes. © 2012 Published by Elsevier B.V. All rights reserved.

Keywords

Biological samples, Capillary zone electrophoresis, Puerarin, Xinkeshu capsules

Publication Date

2012

Source Publication Title

Talanta

Volume

91

Start Page

83

End Page

87

Publisher

Elsevier

DOI

10.1016/j.talanta.2012.01.022

ISSN (print)

00399140

ISSN (electronic)

18733573

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