Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Hollow superparamagnetic iron oxide nanoshells as a hydrophobic anticancer drug carrier: Intracelluar pH-dependent drug release and enhanced cytotoxicity

Language

English

Abstract

With curcumin and doxorubicin (DOX) base as model drugs, intracellular delivery of hydrophobic anticancer drugs by hollow structured superparamagnetic iron oxide (SPIO) nanoshells (hydrodynamic diameter: 191.9 ± 2.6 nm) was studied in glioblastoma U-87 MG cells. SPIO nanoshell-based encapsulation provided a stable aqueous dispersion of the curcumin. After the SPIO nanoshells were internalized by U-87 MG cells, they localized at the acidic compartments of endosomes and lysosomes. In endosome/lysosome-mimicking buffers with a pH of 4.5-5.5, pH-dependent drug release was observed from curcumin or DOX loaded SPIO nanoshells (curcumin/SPIO or DOX/SPIO). Compared with the free drug, the intracellular curcumin content delivered via curcumin/SPIO was 30 fold higher. Increased intracellular drug content for DOX base delivered via DOX/SPIO was also confirmed, along with a fast intracellular DOX release that was attributed to its protonation in the acidic environment. DOX/SPIO enhanced caspase-3 activity by twofold compared with free DOX base. The concentration that induced 50% cytotoxic effect (CC50) was 0.05 ± 0.03 μg ml -1 for DOX/SPIO, while it was 0.13 ± 0.02 μg ml -1 for free DOX base. These results suggested SPIO nanoshells might be a promising intracellular carrier for hydrophobic anticancer drugs. © The Royal Society of Chemistry 2012.

Publication Date

2012

Source Publication Title

Nanoscale

Volume

4

Issue

18

Start Page

5744

End Page

5754

Publisher

Royal Society of Chemistry

DOI

10.1039/c2nr30960b

Link to Publisher's Edition

http://dx.doi.org/10.1039/c2nr30960b

ISSN (print)

20403364

ISSN (electronic)

20403372

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