Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Measurement of yunaconitine and crassicauline A in small-volume blood serum samples by LC–MS/MS: Tracing of aconite poisoning in clinical diagnosis

Language

English

Abstract

Aconite poisoning is one of the most serious types of herb-related medical emergencies. In Hong Kong, many if not most of these poisoning cases are due to confusion in herbal species; that is, the wrong herbs are used in prescriptions. Such human errors, while inevitable perhaps, can be serious, and sometimes fatal. The chemical components responsible for aconite poisoning are yunaconitine and crassicauline A. In the present study, a rapid and sensitive method for the screening and quantification of yunaconitine and crassicauline A in human serum, using LC-MS/MS, was developed and validated. Methyllycaconitine was chosen as the internal standard. The limit of detection (LOD) of yunaconitine and crassicauline A were found to be 0.022 and 0.021 ng/mL, respectively. The limit of quantification (LOQ) was 0.1 ng/mL for both yunaconitine and crassicauline A. The recovery of yunaconitine and crassicauline A ranged from 78.6% to 84.9% and 78.3% to 87.2%, respectively. The matrix effect of yunaconitine and crassicauline A ranged from 110.0% to 130.4% and 121.2 to 130.0%, respectively. Both yunaconitine and crassicauline A were stable in serum for at least 3 months at -20 °C, and the extracts were stable for at least 7 days. For clinical applications, serum samples of two patients confirmed to have had aconite herbs poisoning in 2008 were quantified using the developed method. The result showed that this method can be utilized in clinical routine applications. This screening method expedites the diagnosis in cases of suspected aconite poisoning, thus enabling doctors to treat the condition more quickly and effectively. © 2012 Elsevier B.V. All rights reserved.

Keywords

Crassicauline A, Human serum, LC-MS/MS, Yunaconitine

Publication Date

2012

Source Publication Title

Talanta

Volume

97

Start Page

491

End Page

498

Publisher

Elsevier

DOI

10.1016/j.talanta.2012.05.004

ISSN (print)

00399140

ISSN (electronic)

18733573

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