Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Multifunctional encoded self-assembling protein nanofibrils as platform for high-throughput and multiplexed detection of biomolecules

Language

English

Abstract

A one-dimensional nanofibrillar array formed by the co-assembly of native and biotin-functionalized beta-amyloid (Aβ) peptide was developed for biomolecule sensing. With the presence of biotin moiety, a variety of biomolecular probes can be conjugated onto the nanofibrils, thus converting the protein assembly into a miniature biosensor. In this work, DNA probes were immobilized onto the fibril for the detection of cDNA sequences. The as-developed "DNA-nanoarray" achieved a detection limit at subattomole level (183 fM in 10 μL). This highly sensitive, yet simple, assay requires a trace amount of sample consumption (<10 μL) and is pretreatment-free. In addition, we reported the preparation of alternate-segmented amyloid nanofibrils with multifunctionality. The fibrils hereby serve as an encoded template that can be visualized with various fluorescence labeling dyes for barcode recognition purpose, and, hence, multiplex detection of biomolecules was achieved. Regarding that each protein nanofibril represents a single detection platform, a large number of single fibrils simultaneously are monitored with the dual-color TIRFM in a high-throughput manner. © 2011 American Chemical Society.

Publication Date

2011

Source Publication Title

Analytical Chemistry

Volume

83

Issue

24

Start Page

9370

End Page

9377

Publisher

American Chemical Society

DOI

10.1021/ac2019602

Link to Publisher's Edition

http://dx.doi.org/10.1021/ac2019602

ISSN (print)

00032700

ISSN (electronic)

15206882

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