Document Type

Book Chapter

Department/Unit

Department of Biology

Title

Identification and verification of redox-sensitive proteins in Arabidopsis thaliana

Language

English

Abstract

Environmental stresses often trigger rapid generation of reactive oxygen species (ROS) in plants. Excessive amount of ROS can cause damage to plant cells and thus need to be counteracted by cellular antioxidant systems. On the other hand, ROS also serve as signaling molecules that modulate various physiological responses and developmental processes. Signaling function of ROS is largely achieved through oxidative modifications of redox-sensitive proteins. Therefore, development of methods for high-throughput identification of redox-sensitive proteins and for verifying and characterizing their in vivo redox states is essential for advancing our understanding of ROS-mediated signaling pathways. © 2012 Springer Science+Business Media, LLC.

Keywords

Arabidopsis thaliana, Oxidative stress, Proteomics, Redox-sensitive proteins

Publication Date

2012

Source Publication Title

Plant signalling networks : Methods and protocols

Editors

Wang, Zhi-Yong ; Yang, Zhenbiao

Start Page

83

End Page

94

Series Title

Methods in Molecular Biology: Methods and Protocols

Publisher

Humana Press

DOI

10.1007/978-1-61779-809-2_6

Link to Publisher's Edition

http://dx.doi.org/10.1007/978-1-61779-809-2_6

ISSN (print)

10643745

ISSN (electronic)

19406029

ISBN (print)

9781617798085

ISBN (electronic)

9781617798092

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