Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Solid-phase extraction-field-amplified sample injection coupled with CE-ESI-MS for online pre-concentration and quantitative analysis of brain-gut peptides

Language

English

Abstract

In order to improve the sensitivity of CE-ESI-MS for the analysis of brain-gut peptides, a solid-phase extraction combined with field-amplified sample injection was used for the pre-concentration of the brain-gut peptides. Compared with the conventional pressure injection method, the sensitivity in the detection of brain-gut peptides was improved more than 100-fold. The possible factors affecting sample stacking, such as the sample matrix, the composition and the length of the water column, the types and the volumes of eluent, have been investigated in detail. Under the optimum conditions, the detectable concentration of brain-gut peptides was found to be as low as 0.02μM. A linear response concentration for the detection was developed in the range of 0.08-25μmol/L. A real sample of human cerebrospinal fluids, which was spiked with brain-gut peptides, was also examined in order to evaluate the reliability of the proposed approach. The recovery of the method was in a range from 69.2 to 85.4%. The method was found to be reliable, accurate and potentially applicable for clinical drug analysis. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

Brain-gut peptide, CE-ESI-MS, Cerebrospinal fluid, Field amplified, SPE

Publication Date

2011

Source Publication Title

ELECTROPHORESIS

Volume

32

Issue

20

Start Page

2823

End Page

2829

Publisher

Wiley-VCH Verlag

DOI

10.1002/elps.201000591

Link to Publisher's Edition

http://dx.doi.org/10.1002/elps.201000591

ISSN (print)

01730835

ISSN (electronic)

15222683

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