Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Synthesis of magnetic nanoparticles with immobilized aminophenylboronic acid for selective capture of glycoproteins

Language

English

Abstract

Aminophenylboronic acid (APBA)-functionalized magnetic iron oxide nanoparticles (Fe3O4 MNPs) were synthesized for the selective capture of glycoproteins from unfractionated protein mixtures. The morphology, adsorption, and recognition properties of the resultant particles were investigated and uniform size APBA-coated MNPs with a mean diameter of ∼15 nm and high magnetic saturation value of 30.6 emu g-1 were obtained, which endued the adsorbent with a large surface area and convenience of isolation. The selectivity and binding capacity of APBA-coated MNPs were evaluated by using standard glycoproteins (cellulose and ovalbumin) and nonglycoproteins (bovine hemoglobin, bovine serum albumin and lysozyme) as model samples. Adsorption experiments and SDS-PAGE demonstrated that the APBA-coated MNPs had higher binding capacity and selectivity for glycoproteins compared to nonglycoproteins. In addition, the practicability of the as-prepared MNPs was further assessed by specific capture of ovalbumin from an egg white sample. © 2011 The Royal Society of Chemistry.

Publication Date

2011

Source Publication Title

Journal of Materials Chemistry

Volume

21

Issue

2

Start Page

518

End Page

524

Publisher

Royal Society of Chemistry

DOI

10.1039/C0JM02300K

Link to Publisher's Edition

http://dx.doi.org/10.1039/C0JM02300K

ISSN (print)

09599428

ISSN (electronic)

13645501

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