Document Type

Journal Article

Department/Unit

Department of Physics

Title

Influence of engineered surface on cell directionality and motility

Abstract

Control of cell migration is important in numerous key biological processes, and is implicated in pathological conditions such as cancer metastasis and inflammatory diseases. Many previous studies indicated that cell migration could be guided by micropatterns fabricated on cell culture surfaces. In this study, we designed a polydimethylsiloxane cell culture substrate with gratings punctuated by corners and ends, and studied its effects on the behavior of MC3T3-E1 osteoblast cells. MC3T3-E1 cells elongated and aligned with the gratings, and the migration paths of the cells appeared to be guided by the grating pattern. Interestingly, more than 88% of the cells cultured on these patterns were observed to reverse their migration directions at least once during the 16 h examination period. Most of the reversal events occurred at the corners and the ends of the pattern, suggesting these localized topographical features induce an abrupt loss in directional persistence. Moreover, the cell speed was observed to increase temporarily right after each directional reversal. Focal adhesion complexes were more well-established in cells on the angular gratings than on flat surfaces, but the formation of filipodia appeared to be imbalanced at the corners and the ends, possibly leading to the loss of directional persistence. This study describes the first engineered cell culture surface that consistently induces changes in the directional persistence of adherent cells. This will provide an experimental model for the study of this phenomenon and a valuable platform to control the cell motility and directionality, which can be used for cell screening and selection.

Publication Year

2014

Journal Title

Biofabrication

Volume number

6

Issue number

1

Publisher

IOP Publishing

First Page (page number)

15011

Last Page (page number)

15011

Referreed

1

Funder

The authors would like to acknowledge the financial support from the Center for Biosystems, Neuroscience, and Nanotechnology of City University of Hong Kong under project number 9360148.

DOI

10.1088/1758-5082/6/1/015011

ISSN (print)

17585082

Link to Publisher’s Edition

http://dx.doi.org/10.1088/1758-5082/6/1/015011

Keywords

engineered surface, polydimethylsiloxane molding, directional persistence, cell motility, cell guidance

ISSN (electronic)

17585090

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