Document Type

Journal Article

Department/Unit

School of Chinese Medicine; Department of Chemistry

Abstract

A crude polysaccharide fraction (cDOP) has been determined to be the characteristic marker of Dendrobium officinale, an expensive tea material in Asia, but its chemistry and bioactivity have not been studied. In work reported here, cDOP was destarched (DOP, 90% yield) and separated into two subfraction polysaccharides, DOPa and DOPb, which were characterized by monosaccharide composition and methylation analyses and spectral analyses (FT-IR and 1H and 13C NMR). Both are composed of mannose and glucose at similar ratios and have a similar structure with a backbone of 1,4-linked β-d-mannopyranosyl and β-d-glucopyranosyl residues. Significant differences were observed only in their molecular weights. Bioassay using mouse macrophage cell line RAW264.7 indicated that DOP and its two subfractions enhance cell proliferation, TNF-α secretion, and phagocytosis in a dose-dependent manner. They also induced the proliferation of lymphocytes alone and with mitogens. DOPa and DOPb are thus proven to be major, active polysaccharide markers of D. officinale.

Publication Year

2016

Journal Title

Journal of Agricultural and Food Chemistry

Volume number

64

Issue number

4

Publisher

American Chemical Society

First Page (page number)

881

Last Page (page number)

889

Referreed

1

DOI

10.1021/acs.jafc.5b05180

ISSN (print)

00218561

Keywords

chemical structure, Dendrobium officinale, lymphocytes, polysaccharides, RAW264.7 cells

ESSN

15205118

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