School of Chinese Medicine
A delivery system specifically approaching bone resorption surfaces to facilitate therapeutic modulation of microRNAs in osteoclasts
Dysregulated microRNAs in osteoclasts could cause many skeletal diseases. The therapeutic manipulation of these pathogenic microRNAs necessitates novel, efficient delivery systems to facilitate microRNAs modulators targeting osteoclasts with minimal off-target effects. Bone resorption surfaces characterized by highly crystallized hydroxyapatite are dominantly occupied by osteoclasts. Considering that the eight repeating sequences of aspartate (D-Asp8) could preferably bind to highly crystallized hydroxyapatite, we developed a targeting system by conjugating D-Asp8 peptide with liposome for delivering microRNA modulators specifically to bone resorption surfaces and subsequently encapsulated antagomir-148a (a microRNA modulator suppressing the osteoclastogenic miR-148a), i.e. (D-Asp8)-liposome-antagomir-148a. Our results demonstrated that D-Asp8 could facilitate the enrichment of antagomir-148a and the subsequent down-regulation of miR-148a in osteoclasts in vivo, resulting in reduced bone resorption and attenuated deterioration of trabecular architecture in osteoporotic mice. Mechanistically, the osteoclast-targeted delivery depended on the interaction between bone resorption surfaces and D-Asp8. No detectable liver and kidney toxicity was found in mice after single/multiple dose(s) treatment of (D-Asp8)-liposome-antagomir-148a. These results indicated that (D-Asp8)-liposome as a promising osteoclast-targeting delivery system could facilitate clinical translation of microRNA modulators in treating those osteoclast-dysfunction-induced skeletal diseases.
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This work was supported by the Faculty Research Grant of Hong Kong Baptist University (FRG2/13-14/006), the Hong Kong General Research Fund (12102914 and HKBU 478312), Interdisciplinary Research Matching Scheme (IRMS) of Hong Kong Baptist University (RC-IRMS/13-14/02 and RC-IRMS/13-14/03), the Research Committee of Hong Kong Baptist University (30-12-286 and FRG2/12-13/027), the Science and Technology Innovation Commission of Shenzhen Municipality (SCM-2013-SZTIC-001), Natural Science Foundation Council (81272045 and 81401833).
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Targeted delivery system, MicroRNA, Osteoclast, Bone resorption
Liu, Jin, Lei Dang, Defang Li, Chao Liang, Xiaojuan He, Heng Wu, Airong Qian, Zhijun Yang, Doris W. T. Au, Michael W. L. Chiang, Bao-Ting Zhang, Quanbin Han, Kin Man Kevin Yue, Hong Qi Zhang, Changwei Lv, Xiaohua Pan, Jiake Xu, Zhaoxiang Bian, Peng Shang, Weihong Tan, Zicai Liang, Baosheng Guo, Aiping Lu, and Ge Zhang. "A delivery system specifically approaching bone resorption surfaces to facilitate therapeutic modulation of microRNAs in osteoclasts." Biomaterials 52 (2015): 148-160.