Document Type

Journal Article

Department/ Unit

School of Chinese Medicine

Title

Gambogenic acid inhibits LPS-simulated inflammatory response by suppressing NF-κB and MAPK in macrophages

Abstract

Inflammation is a response of body tissues to injury and infection. Compounds that can inhibit inflammation have been shown to have potential therapeutic clinical application. Gambogenic acid (GEA) has potent antitumor and anti- inflammatory activities. Herein, the molecular mechanisms of GEA's anti-inflammatory effect were investigated in lipopolysaccharide (LPS)-stimulated macrophage cells. The results showed that pretreatment with GEA could markedly inhibit interleukin (IL)-1α, IL-1β, tumor necrosis factor-α, IFN-β, IL-12b, and IL-23a production in a dose-dependent manner in LPS-induced model. Furthermore, this drug significantly reduced the release of nitric oxide (NO), and impaired the protein level of inducible NO synthase and the cyclooxygenase 2. The finding also showed that the effect of GEA may be related to the suppression of the nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathway. These results indicate that GEA could suppress LPS-simulated inflammatory response partially by attenuating NO synthesis and NF-κB and MAPK activation, suggesting that it may become a potent therapeutic agent for the treatment of inflammatory diseases.

Publication Year

2016

Journal Title

Acta Biochimica et Biophysica Sinica

Volume number

48

Issue number

5

Publisher

Oxford University Press

First Page (page number)

451

Last Page (page number)

461

Referreed

1

DOI

10.1093/abbs/gmw021

ISSN (print)

16729145

Link to Publisher’s Edition

http://dx.doi.org/10.1093/abbs/gmw021

Keywords

inflammation, gambogenic acid, NO NF-κB pathway, MAPK pathway

This document is currently not available here.

Share

COinS