Document Type

Journal Article

Department/ Unit

School of Chinese Medicine

Title

A tutorial review for employing enzymes for the construction of G-quadruplex-based sensing platforms

Abstract

With rapid advances in the field of DNA chemistry, nucleic acids and DNA-modifying enzymes have recently emerged as versatile components for the construction of oligonucleotide-based sensors. Meanwhile, the G-quadruplex motif has been widely employed for the development of DNA-based assays due to its diverse structural variety. In this tutorial, we introduce the principles of G-quadruplex-based sensing and the use of DNA-modifying enzymes for sensor platform development. We also highlight recent studies of the application of DNA-modifying enzymes for the development of G-quadruplex-based luminescent detection platforms with a view towards how those enzymes play an important role in sensitivity enhancement.

Publication Year

2016

Journal Title

Analytica Chimica Acta

Volume number

913

Publisher

Elsevier Masson

First Page (page number)

41

Last Page (page number)

54

Referreed

1

DOI

10.1016/j.aca.2016.01.043

ISSN (print)

00032670

Link to Publisher’s Edition

http://dx.doi.org/10.1016/j.aca.2016.01.043

Keywords

Oligonucleotide-based sensor, G-quadruplex, Enzyme, Sensor construction

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