Document Type

Journal Article

Department/ Unit

School of Chinese Medicine

Abstract

A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)– and (1→6)–Glcp, bearing terminal- and (1→3)–Glcp side-chains at O-3 position of (1→6)–Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.

Publication Year

2014

Journal Title

PLoS ONE

Volume number

9

Issue number

7

Publisher

Public Library of Science

First Page (page number)

e100380

Referreed

1

DOI

10.1371/journal.pone.0100380

ESSN

19326203

Additional Files

JA-5037-28271_publisher_suppl.doc (107 kB)

Share

COinS