Document Type

Journal Article

Department/ Unit

School of Chinese Medicine

Title

Isolation, structure characterization, and immunomodulating activity of a hyperbranched polysaccharide from the fruiting bodies of Ganoderma sinense

Abstract

A polysaccharide (GSP-6B) with a molecular mass of 1.86 × 106 Da was isolated from the fruiting bodies of Ganoderma sinense. Chemical composition analysis, methylation analysis, infrared spectroscopy, and nuclear magnetic resonance spectroscopy were conducted to elucidate its structure. GSP-6B contains a backbone of (1→6)-linked-β-d-glucopyranosyl residues, bearing branches at the O-3 position of every two sugar residues along the backbone. The side chains contain (1→4)-linked-β-d-glucopyranosyl residues, (1→3)-linked-β-d-glucopyranosyl residues, and nonreducing end β-d-glucopyranosyl residues. An in vitro immunomodulating activity assay revealed that GSP-6B could significantly induce the release of IL-1β and TNF-α in human peripheral blood mononuclear cell (PBMC) and showed no toxicity to either PBMC or a human macrophage cell line THP-1. GSP-6B could also activate dendritic cells (DC) by stimulating the secretion of IL-12 and IL-10 from DC.

Publication Year

2012

Journal Title

Journal of Agricultural and Food Chemistry

Volume number

60

Issue number

17

Publisher

American Chemical Society

First Page (page number)

4276

Last Page (page number)

4281

Referreed

1

DOI

10.1021/jf205056u

ISSN (print)

00218561

Keywords

cytokine, dendritic cell, Ganoderma sinense, immunological activity, polysaccharide

ESSN

15205118

This document is currently not available here.

Share

COinS