Document Type

Journal Article

Department/ Unit

School of Chinese Medicine

Title

Separation, structure characterization, conformation and immunomodulating effect of a hyperbranched heteroglycan from Radix Astragali

Abstract

A water soluble polysaccharide (RAP) was isolated and purified from Radix Astragali and its structure was elucidated by monosaccharide composition, partial acid hydrolysis and methylation analysis, and further supported by FT-IR, GC–MS and 1H and 13C NMR spectra, SEM and AFM microscopy. Its average molecular weight was 1334 kDa. It was composed of Rha, Ara, Glc, Gal and GalA in a molar ratio of 0.03:1.00:0.27:0.36:0.30. The backbone consisted of 1,2,4-linked Rhap, α-1,4-linked Glcp, α-1,4-linked GalAp6Me, β-1,3,6-linked Galp, with branched at O-4 of the 1,2,4-linked Rhap and O-3 or O-4 of β-1,3,6-linked Galp. The side chains mainly consisted of α-T-Araf and α-1,5-linked Araf with O-3 as branching points, having trace Glc and Gal. The terminal residues were T-linked Araf, T-linked Glcp and T-linked Galp. Morphology analysis showed that RAP took random coil feature. RAP exhibited significant immunomodulating effects by stimulating the proliferation of human peripheral blood mononuclear cells and enhancing its interleukin production.

Publication Year

2012

Journal Title

Carbohydrate Polymers

Volume number

87

Issue number

1

Publisher

Elsevier

First Page (page number)

667

Last Page (page number)

675

Referreed

1

DOI

10.1016/j.carbpol.2011.08.045

ISSN (print)

01448617

Keywords

Radix Astragali, Polysaccharide, Structure character, Morphology feature, Immunomodulating effect

ESSN

18791344

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