School of Chinese Medicine
Separation, structure characterization, conformation and immunomodulating effect of a hyperbranched heteroglycan from Radix Astragali
A water soluble polysaccharide (RAP) was isolated and purified from Radix Astragali and its structure was elucidated by monosaccharide composition, partial acid hydrolysis and methylation analysis, and further supported by FT-IR, GC–MS and 1H and 13C NMR spectra, SEM and AFM microscopy. Its average molecular weight was 1334 kDa. It was composed of Rha, Ara, Glc, Gal and GalA in a molar ratio of 0.03:1.00:0.27:0.36:0.30. The backbone consisted of 1,2,4-linked Rhap, α-1,4-linked Glcp, α-1,4-linked GalAp6Me, β-1,3,6-linked Galp, with branched at O-4 of the 1,2,4-linked Rhap and O-3 or O-4 of β-1,3,6-linked Galp. The side chains mainly consisted of α-T-Araf and α-1,5-linked Araf with O-3 as branching points, having trace Glc and Gal. The terminal residues were T-linked Araf, T-linked Glcp and T-linked Galp. Morphology analysis showed that RAP took random coil feature. RAP exhibited significant immunomodulating effects by stimulating the proliferation of human peripheral blood mononuclear cells and enhancing its interleukin production.
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Radix Astragali, Polysaccharide, Structure character, Morphology feature, Immunomodulating effect
Yin, Jun-Yi, Ben Chung-Lap Chan, Hua Yu, Iris Yuen-Kam Lau, Xiao-Qiang Han, Sau-Wan Cheng, Chun-Kwok Wong, Clara Bik-San Lau, Ming-Yong Xie, Kwok-Pui Fung, Ping-Chung Leung, and Quan-Bin Han. "Separation, structure characterization, conformation and immunomodulating effect of a hyperbranched heteroglycan from Radix Astragali." Carbohydrate Polymers 87.1 (2012): 667-675.
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