Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

A bioactivity-guided study on the anti-diarrheal activity of Polygonum chinense Linn

Abstract

Ethnopharmacological relevance

Polygonum chinense Linn., a folk medicine, has long been used for the treatment of diarrhea and enteritis in southwestern China. However, the components responsible for its anti-diarrheal activity are still poorly understood.

Aim of the study

To determine anti-diarrheal activities of Polygonum chinense L. and to identify its active components through bioactivity-guided isolation technique.

Materials and methods

Animals were orally administered with the extract of Polygonum chinense L. The anti-diarrheal effects of 75% ethanol extract, four fractions with different polarities from 75% ethanol extract, different eluates collected from Diaion HP-20 macroporous resin chromatography, ellagic acid and corilagin, were examined based on mouse models of castor oil- and magnesium sulfate-induced diarrhea.

Results

The results showed that the 75% ethanol extract of Polygonum chinense L. exhibited significant anti-diarrheal activities in a dose-dependent manner in two mouse models. Through in vivo bioactivity-guided fractionation processes, n-butanol and aqueous fractions were found to exhibit prominent anti-diarrheal activities, and two major compounds, ellagic acid and corilagin, from these active fractions were found to possess anti-diarrheal effects.

Conclusion

Present study provides evidence of the utilization of Polygonum chinense L. for diarrhea, and ellagic acid and corilagin are two components contributing to the anti-diarrheal effect.

Publication Year

2013

Journal Title

Journal of Ethnopharmacology

Volume number

149

Issue number

2

Publisher

Elsevier

First Page (page number)

499

Last Page (page number)

505

Referreed

1

Funder

The study was supported by research grants FRG-II/10–11/027 from Hong Kong Baptist University. We thank Dr. Xian-Wen Yang (Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences) for providing standard marker of ellagic acid. We also thank Mr. Hing-Man Ho (School of Chinese medicine, Hong Kong Baptist University) for technical support in LC/MS-MS analysis.

DOI

10.1016/j.jep.2013.07.007

ISSN (print)

03788741

Keywords

Polygonum chinense Linn., Anti-diarrheal activity, Ellagic acid, Corilagin

ESSN

18727573

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