School of Chinese Medicine
A bioactivity-guided study on the anti-diarrheal activity of Polygonum chinense Linn
Polygonum chinense Linn., a folk medicine, has long been used for the treatment of diarrhea and enteritis in southwestern China. However, the components responsible for its anti-diarrheal activity are still poorly understood.
Aim of the study
To determine anti-diarrheal activities of Polygonum chinense L. and to identify its active components through bioactivity-guided isolation technique.
Materials and methods
Animals were orally administered with the extract of Polygonum chinense L. The anti-diarrheal effects of 75% ethanol extract, four fractions with different polarities from 75% ethanol extract, different eluates collected from Diaion HP-20 macroporous resin chromatography, ellagic acid and corilagin, were examined based on mouse models of castor oil- and magnesium sulfate-induced diarrhea.
The results showed that the 75% ethanol extract of Polygonum chinense L. exhibited significant anti-diarrheal activities in a dose-dependent manner in two mouse models. Through in vivo bioactivity-guided fractionation processes, n-butanol and aqueous fractions were found to exhibit prominent anti-diarrheal activities, and two major compounds, ellagic acid and corilagin, from these active fractions were found to possess anti-diarrheal effects.
Present study provides evidence of the utilization of Polygonum chinense L. for diarrhea, and ellagic acid and corilagin are two components contributing to the anti-diarrheal effect.
Journal of Ethnopharmacology
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The study was supported by research grants FRG-II/10–11/027 from Hong Kong Baptist University. We thank Dr. Xian-Wen Yang (Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences) for providing standard marker of ellagic acid. We also thank Mr. Hing-Man Ho (School of Chinese medicine, Hong Kong Baptist University) for technical support in LC/MS-MS analysis.
Polygonum chinense Linn., Anti-diarrheal activity, Ellagic acid, Corilagin
Xiao, Hai-Tao, Siu-Wai Tsang, Hong-Yan Qin, Franky F. K. Choi, Zhijun Yang, Quan-Bin Han, Hubiao Chen, Hong-Xi Xu, Hong Shen, Aiping Lu, and Zhaoxiang Bian. "A bioactivity-guided study on the anti-diarrheal activity of Polygonum chinense Linn." Journal of Ethnopharmacology 149.2 (2013): 499-505.
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