Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Abstract

Background: Eruberin A (2, 3-dehydroflavonoid), a flavanol glycoside isolated from Pronephrium penangianum, has been used as a blood-nourishing folk medicine for centuries; however, it indeed possesses a variety of other health-promoting benefits including antifibrotic bioactivity. Activation of pancreatic stellate cells (PSCs) is the key initiating step in pancreatic fibrosis, which is a characteristic feature associated with chronic pancreatitis and pancreatic adenocarcinoma. Methods: The anti-fibrotic effect of eruberin A and the underlying mechanisms of its anti-fibrotic action in LTC-14 cells, which retained essential characteristics and morphological features of primary PSCs, were examined by means of real-time polymerase chain reactions, Western blotting and immunostaining. Results: The application of eruberin A (20 μg/ml) effectively inhibited the expression levels of fibrotic mediators namely alphasmooth muscle actin, fibronectin and type I-collagen, so as the sonic hedgehog signaling pathway components post transforming growth factor-beta (5 ng/ml) stimulation. Eruberin A treatment also led to a notable decrease in the activation of nuclear factor-kappaB (NF-κB) and the phosphorylation of phosphoinositide 3-kinase (PI3K)/serine-threonine kinase (AKT). Conclusion: Our results demonstrated that eruberin A significantly suppressed the expression levels of fibrotic mediators in PSCs, and we suggest that its anti-fibrotic mechanism was associated with an attenuation of the PI3K/AKT/NF-κB signaling pathway.

Publication Year

2015

Journal Title

Cellular Physiology and Biochemistry

Volume number

6

Issue number

36

Publisher

Karger Publishers Open Access

First Page (page number)

2433

Last Page (page number)

2446

Referreed

1

Funder

This work was supported by the National Natural Science Foundation of China (Young Scientist Project #81400666) and Hong Kong Baptist University (Interdisciplinary Research Matching Scheme #RC-IRMS/12-13/03).

DOI

10.1159/000430204

ISSN (print)

10158987

Link to Publisher’s Edition

http://dx.doi.org/10.1159/000430204

Copyright

This is an Open Access article licensed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported license (CC BY-NC) (www.karger.com/OA-license), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only.

Keywords

Alpha-smooth muscle actin, Nuclear factor-kappaB, Pancreatic fibrosis, Pancreatic stellate cells, PI3K/AKT, Sonic hedgehog

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