Structure elucidation and immunomodulatory activity of a beta glucan from the fruiting bodies of Ganoderma sinense

Xiao-Qiang Han, State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, Chinese University of Hong Kong
Gar-Lee Yue, State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, Chinese University of Hong Kong
Rui-Qi Yue, School of Chinese Medicine, Hong Kong Baptist University
Cai-Xia Dong, School of Chinese Medicine, Hong Kong Baptist University
Chung-Lap Chan, State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, Chinese University of Hong Kong
Chun-Hay Ko, State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, Chinese University of Hong Kong
Wing-Shing Cheung, State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, Chinese University of Hong Kong
Ke-Wang Luo, State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, Chinese University of Hong Kong
Hui Dai, Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center
Chun-Kwok Wong, State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, Chinese University of Hong Kong
Ping-Chung Leung, State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, Chinese University of Hong Kong
Quan-Bin Han, State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, Chinese University of Hong Kong

Abstract

A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells. © 2014 Han et al.