Document Type

Journal Article

Department/Unit

School of Chinese Medicine

Title

Anti-inflammatory actions of (+)-3′α-angeloxy-4′-keto-3′,4′-dihydroseselin (Pd-Ib) against dextran sulfate sodium-induced colitis in C57BL/6 mice

Abstract

The immunoregulatory protective properties of (+)-3′α-angeloxy-4′-keto-3′,4′-dihydroseselin (Pd-Ib) isolated from Bupleurum malconense has not been reported. In the present study, the therapeutic effect of Pd-Ib (30, 60, and 120 mg/kg/day) was examined in a mouse model of dextran sulfate sodium (DSS)-induced acute colitis. Administration of Pd-Ib significantly reduced the disease activity index, inhibited the shortening of colon length, reduced colonic tissue damage, and suppressed colonic myeloperoxidase activity and nitric oxide levels in mice with DSS-induced colitis. Moreover, Pd-Ib greatly suppressed the secretion of pro-inflammatory cytokines TNF-α, IFN-γ, IL-6, and IL-17A while enhancing the level of anti-inflammatory cytokine IL-4. The protein levels of phosphorylated STAT3 (p-STAT3) and phosphorylated p38 (p-p38) were down-regulated in the colonic tissues of DSS-treated mice. Importantly, the anti-inflammatory effect of Pd-Ib against acute colitis was comparable to the anti-inflammatory sulfa drug sulfasalazine (300 mg/kg). Furthermore, the in vitro study showed that the inhibitory effect of Pd-Ib on p-STAT3 and IL-6 protein levels was accompanied by the reduction of MAPKs (JNK and p38). In conclusion, this study suggested that Pd-Ib attenuated DSS-induced acute colitis via the regulation of interleukins principally through the STAT3 and MAPK pathways.

Publication Year

2016

Journal Title

Journal of Natural Products

Volume number

79

Issue number

4

Publisher

American Chemical Society

First Page (page number)

1056

Last Page (page number)

1062

Referreed

1

DOI

10.1021/acs.jnatprod.5b01071

ISSN (print)

01633864

Link to Publisher’s Edition

http://dx.doi.org/10.1021/acs.jnatprod.5b01071

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