Department of Biology
Ginsenosides, the active components of the famous Chinese herb, ginseng, has been suggested to possess cardio-protective effects. The mechanism of ginsenosides is believed to associate with their ability to prevent cellular oxidative stress. The purpose of this study is to explore the cyto-protective effect of ginsenoside protopanaxatriol (PPT) on hydrogen peroxide (H2O2) induced endothelial cell injury and cell death. Pre-treatment of the human umbilical vein endothelial cells (HUVECs) with PPT for 24 hours was found to protect cells against the toxicity of H2O2. Besides, it can also reduce H2O2-induced DNA damage. H2O2 treatment induced an over-activation of the DNA repair enzyme PARP-1 and concomitant depletion of intracellular substrate NAD+, were reduced by the pre-treatment of PPT. PPT could also reverse the decrease in ATP/ADP ratio caused by H2O2. While the GSH depletion in oxidative stressed HUVECs was found to be restored through enhancing the activities of glutathione reductase and glutathione peroxidase. The present findings suggest that ginsenoside PPT could protect HUVECs against H2O2-induced injury via modulating intracellular redox status. These results implicated that the protective capability of PPT on endothelial cells against oxidative stress may be responsible for the cardio-protective action of ginseng.
Ginsenoside, protopanaxatriol, endothelial cell, oxidative stress, hydrogen peroxide, cellular redox status, free radcials
Source Publication Title
Free Radical Biology & Medicine
Link to Publisher's Edition
Kowk, HH, WY Ng, MS Yang, NK Mak, RNS Wong, and PYK Yue. "The ginsenoside protopanaxatriol protects endothelial cells from hydrogen peroxide-induced cell injury and cell death by modulating intracellualr redox status." Free Radical Biology & Medicine 48 (2010): 437-445.