Department of Chemistry
A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe
An iridium(III) complex exhibiting higher responce towards i-motif DNA over dsDNA and ssDNA was employed for the construction of a TdT activity detection platform. The assay exhibited a linear signal enhancement for TdT in the concentration range of 0 to 8 U mL−1, and the limit of detection for TdT was 0.25 U mL−1.
Source Publication Title
Royal Society of Chemistry
Link to Publisher's Edition
Lu, Lihua, Modi Wang, Li-Juan Liu, Chun-Yuen Wong, Chung-Hang Leung, and Dik Lung Ma. "A luminescence switch-on probe for terminal deoxynucleotidyl transferase (TdT) activity detection by using an iridium(III)-based i-motif probe." Chemical Communications 51.49 (2015): 9953-9956.