Document Type

Journal Article

Title

A luminescent cocaine detection platform using a split G-quadruplex-selective iridium(III) complex and a three-way DNA junction architecture

Department/Unit

Department of Chemistry

Language

English

Abstract

In this study, a series of 10 in-house cyclometalated iridium(III) complexes bearing different auxiliary ligands were tested for their selectivity toward split G-quadruplex in order to construct a label-free switch-on cocaine detection platform employing a three-way junction architecture and a G-quadruplex motif as a signal output unit. Through two rounds of screening, we discovered that the iridium(III) complex 7 exhibited excellent selectivity toward the intermolecular G-quadruplex motif. A detection limit as low as 30 nM for cocaine can be achieved by this sensing approach with a linear relationship between luminescence intensity and cocaine concentration established from 30 to 300 nM. Furthermore, this sensing approach could detect cocaine in diluted oral fluid. We hope that our simple, signal-on, label-free oligonucleotide-based sensing method for cocaine using a three-way DNA junction architecture could act as a useful platform in bioanalytical research.

Keywords

cocaine, G-quadruplex, iridium(III) complex, label-free, three-way junction

Publication Date

9-30-2015

Source Publication Title

ACS Applied Materials and Interfaces

Volume

7

Issue

34

Start Page

19060

End Page

19067

Publisher

American Chemical Society

Peer Reviewed

1

DOI

10.1021/acsami.5b05861

Link to Publisher's Edition

http://dx.doi.org/10.1021/acsami.5b05861

ISSN (print)

19448244

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