Document Type

Journal Article

Department/Unit

Department of Chemistry

Title

Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex

Language

English

Abstract

In this study, we synthesized a series of 9 luminescent iridium(III) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(III) complex 8 [Ir(pbtz)2(dtbpy)]PF6 (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.

Publication Date

11-30-2015

Source Publication Title

Chemical Communications

Volume

51

Issue

89

Start Page

16033

End Page

16036

Publisher

Royal Society of Chemistry

Peer Reviewed

1

DOI

10.1039/C5CC06655G

Link to Publisher's Edition

http://dx.doi.org/10.1039/C5CC06655G

ISSN (print)

13597345

This document is currently not available here.

Share

COinS