Department of Chemistry
Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex
In this study, we synthesized a series of 9 luminescent iridium(III) complexes and studied their ability to function as luminescent probes for G-quadruplex DNA. The iridium(III) complex 8 [Ir(pbtz)2(dtbpy)]PF6 (where pbtz = 2-phenylbenzo[d]thiazole; dtbpy = 4,4′-di-tert-butyl-2,2′-bipyridine) showed high selectivity for G-quadruplex DNA over single-stranded and double-stranded DNA, and was subsequently utilized for the development of a label-free oligonucleotide-based assay for interferon-gamma (IFN-γ), an important biomarker for a range of immune and infectious diseases, in aqueous solution. We further demonstrated that this assay could monitor IFN-γ levels even in the presence of cellular debris. This assay represents the first G-quadruplex-based assay for IFN-γ detection described in the literature.
Royal Society of Chemistry
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Lin, Sheng, Bingyong He, Chao Yang, Chung-Hang Leung, Jean-Louis Mergny, and Dik Lung Ma. "Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex." Chemical Communications 51.89 (2015): 16033-16036.