Department of Chemistry
We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This ‘mix-and-detect’ methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+.
iridium(III), exonuclease III, G-quadruplex, mercury(II) ion
Source Publication Title
Science and Technology of Advanced Materials
National Institute for Materials Science
Link to Publisher's Edition
Wang, W., Kang, T., Chan, P., Lu, J., Chen, X., Leung, C., & Ma, D. (2015). A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T–Hg2+–T mismatched DNA. Science and Technology of Advanced Materials, 16 (6). https://doi.org/10.1088/1468-6996/16/6/065004