Author

Sheng Lin

Year of Award

3-21-2019

Degree Type

Thesis

Degree Name

Doctor of Philosophy (PhD)

Department

Department of Chemistry.

Principal Supervisor

Ma, Edmond Dik Lung

Keywords

Biochemical markers;Iridium.

Language

English

Abstract

Luminescent transition metal complexes have arisen as viable alternatives to organic dyes for sensory applications due to their notable advantages. This thesis aimed to synthesize different kinds of iridium(III) complexes, explore their interactions with DNAs and investigate their application for the construction of oligonucleotide-based sensing platforms for important biomarkers. A series of iridium(III) complexes incorporating a variety of C^N and N^N donor ligands were synthesized and were demonstrated to possess G-quadruplex-selective binding properties via emission titration, UV/vis titration, fluorescence resonance energy transfer melting and G-quadruplex fluorescent intercalator displacement experiments. These G-quadruplex-selective iridium(III) complexes were utilized as signal transducers to monitor the conformational changes of oligonucleotides in oligonucleotide-based luminescent detection platforms for protein tyrosine kinase-7, interferon-gamma, sialic acidbinding immunoglobulin-likelectin-5 and thymine DNA glycosylase. And these designed platforms could work effectively in the diluted cell extract as the results in this thesis indicated.

Comments

Principal supervisor: Dr. Ma Edmond Dik Lung ; Thesis submitted to the Department of Chemistry ; Thesis (Ph.D.)--Hong Kong Baptist University, 2019.

Bibliography

Includes bibliographical references (pages 172-177).

Available for download on Friday, July 23, 2021



Share

COinS