Document Type

Journal Article

Department/Unit

Department of Biology

Title

Photodynamic therapy-mediated modulation of inflammatory cytokine production by Epstein–Barr virus-infected nasopharyngeal carcinoma cells

Language

English

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant disease associated with Epstein-Barr virus (EBV) infection. This study aims to examine the effects of EBV infection on the production of proinflammatory cytokines in NPC cells after the Zn-BC-AM photodynamic therapy (PDT) treatment. Cells were treated with the photosensitiser Zn-BC-AM for 24 h before light irradiation. Quantitative ELISA was used to evaluate the production of cytokines. Under the same experimental condition, HK-1-EBV cells produced a higher basal level of IL-1α (1561 pg/ml), IL-1Β (16.6 pg/ml) and IL-8 (422.9 pg/ml) than the HK-1 cells. At the light dose of 0.25-0.5 J/cm 2, Zn-BC-AM PDT-treated HK-1-EBV cells were found to produce a higher level of IL-1α and IL-1Β than the HK-1 cells. The production of IL-1Β appeared to be mediated via the IL-1Β-converting enzyme (ICE)-independent pathway. In contrast, the production of angiogenic IL-8 was downregulated in both HK-1 and HK-1-EBV cells after Zn-BC-AM PDT. Our results suggest that Zn-BC-AM PDT might indirectly reduce tumour growth through the modulation of cytokine production. © 2010 CSI and USTC. All rights reserved.

Keywords

cytokines, Epstein-Barr virus, nasopharyngeal carcinoma, Photodynamic therapy

Publication Date

2010

Source Publication Title

Cellular and Molecular Immunology

Volume

7

Issue

4

Start Page

323

End Page

326

Publisher

Nature Publishing Group

DOI

10.1038/cmi.2010.4

Link to Publisher's Edition

http://dx.doi.org/10.1038/cmi.2010.4

ISSN (print)

16727681

ISSN (electronic)

20420226

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