Document Type
Journal Article
Department/Unit
Department of Biology
Title
Photodynamic therapy-mediated modulation of inflammatory cytokine production by Epstein–Barr virus-infected nasopharyngeal carcinoma cells
Language
English
Abstract
Nasopharyngeal carcinoma (NPC) is a malignant disease associated with Epstein-Barr virus (EBV) infection. This study aims to examine the effects of EBV infection on the production of proinflammatory cytokines in NPC cells after the Zn-BC-AM photodynamic therapy (PDT) treatment. Cells were treated with the photosensitiser Zn-BC-AM for 24 h before light irradiation. Quantitative ELISA was used to evaluate the production of cytokines. Under the same experimental condition, HK-1-EBV cells produced a higher basal level of IL-1α (1561 pg/ml), IL-1Β (16.6 pg/ml) and IL-8 (422.9 pg/ml) than the HK-1 cells. At the light dose of 0.25-0.5 J/cm 2, Zn-BC-AM PDT-treated HK-1-EBV cells were found to produce a higher level of IL-1α and IL-1Β than the HK-1 cells. The production of IL-1Β appeared to be mediated via the IL-1Β-converting enzyme (ICE)-independent pathway. In contrast, the production of angiogenic IL-8 was downregulated in both HK-1 and HK-1-EBV cells after Zn-BC-AM PDT. Our results suggest that Zn-BC-AM PDT might indirectly reduce tumour growth through the modulation of cytokine production. © 2010 CSI and USTC. All rights reserved.
Keywords
cytokines, Epstein-Barr virus, nasopharyngeal carcinoma, Photodynamic therapy
Publication Date
2010
Source Publication Title
Cellular and Molecular Immunology
Volume
7
Issue
4
Start Page
323
End Page
326
Publisher
Nature Publishing Group
DOI
10.1038/cmi.2010.4
Link to Publisher's Edition
http://dx.doi.org/10.1038/cmi.2010.4
ISSN (print)
16727681
ISSN (electronic)
20420226
APA Citation
Koon, H., Lo, K., Leung, K., Lung, M., Chang, C., Wong, R., Leung, W., & Mak, N. (2010). Photodynamic therapy-mediated modulation of inflammatory cytokine production by Epstein–Barr virus-infected nasopharyngeal carcinoma cells. Cellular and Molecular Immunology, 7 (4), 323-326. https://doi.org/10.1038/cmi.2010.4