Department of Chemistry
Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe
We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(iii) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated. © 2013 The Royal Society of Chemistry.
Source Publication Title
Royal Society of Chemistry
Link to Publisher's Edition
Leung, K., He, H., Ma, V., Zhong, H., Chan, D., Zhou, J., Mergny, J., Leung, C., & Ma, D. (2013). Detection of base excision repair enzyme activity using a luminescent G-quadruplex selective switch-on probe. Chemical Communications, 49 (50), 5630-5632. https://doi.org/10.1039/c3cc41129j