Department of Chemistry
A label-free luminescent switch-on assay for atp using a g-quadruplex-selective iridium(III) complex
We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5′-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 μM. © 2013 Leung et al.
Source Publication Title
Public Library of Science
Link to Publisher's Edition
Leung, K., Lu, L., Wang, M., Mak, T., Chan, D., Tang, F., Leung, C., Kwan, H., Yu, Z., & Ma, D. (2013). A label-free luminescent switch-on assay for atp using a g-quadruplex-selective iridium(III) complex. PLoS ONE, 8 (10). https://doi.org/10.1371/journal.pone.0077021