Department of Chemistry
Label-free luminescent switch-on detection of endonuclease iv activity using a G-quadruplex-selective iridium(III) complex
We report herein the synthesis and application of a novel G-quadruplex-selective luminescent iridium(III) complex [Ir(ppy) 2(bcp)]+ (where ppy = 2-phenylpyridine and bcp = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline) for the sensitive detection of apurinic/apyrimidinic (AP) endonuclease activity. Using endonuclease IV (Endo IV) as a model enzyme, a duplex DNA substrate containing a G-quadruplex-forming sequence is cleaved by Endo IV at the abasic site. This releases the G-quadruplex sequence, which folds into a G-quadruplex and is recognised by the G-quadruplex-selective iridium(III) complex with an enhanced luminescence response. The assay achieved high sensitivity and selectivity for Endo IV over other tested enzymes. © 2013 American Chemical Society.
AP endonuclease, G-quadruplex, iridium(III) complex, label-free, luminescent
Source Publication Title
ACS Applied Materials and Interfaces
American Chemical Society
Link to Publisher's Edition
Leung, Ka-Ho, Hong-Zhang He, Wei Wang, Hai-Jing Zhong, Daniel Shiu-Hin Chan, Chung-Hang Leung, and Dik-Lung Ma. "Label-free luminescent switch-on detection of endonuclease iv activity using a G-quadruplex-selective iridium(III) complex." ACS Applied Materials and Interfaces 5.23 (2013): 12249-12253.