Department of Chemistry
Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe
A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity. © the Partner Organisations 2014.
Source Publication Title
Royal Society of Chemistry
Link to Publisher's Edition
He, H., Leung, K., Wang, W., Chan, D., Leung, C., & Ma, D. (2014). Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe. Chemical Communications, 50 (40), 5313-5315. https://doi.org/10.1039/C3CC47444E