School of Chinese Medicine
An interesting two-phase solvent system and its use in preparative isolation of aconitines from aconite roots by counter-current chromatography
Two-phase solvent system plays crucial role in successful separation of organic compounds using counter-current chromatography (CCC). An interesting two-phase solvent system, composed of chloroform/ethyl acetate/methanol/water, is reported here, in which both phases contain sufficient organic solvents to balance their dissolving capacities. Adjusting the solvent system to get satisfactory partition coefficients (K values) for target compounds becomes relatively simple. This solvent system succeeded in sample preparation of aconitine (8.07 mg, 93.69%), hypaconitine (7.74 mg, 93.17%), mesaconitine (1.95 mg, 94.52%) from raw aconite roots (102.24 mg, crude extract), benzoylmesaconine (34.79 mg, 98.67%) from processed aconite roots (400.01 mg, crude extract), and yunaconitine (253.59 mg, 98.65%) from a crude extract of Aconitum forrestii (326.69 mg, crude extract). © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Aconitine, Counter-current chromatography, Two-phase solvent system
Source Publication Title
Journal of Separation Science
Link to Publisher's Edition
Han, Q., Tang, W., Dong, C., Xu, H., & Jiang, Z. (2013). An interesting two-phase solvent system and its use in preparative isolation of aconitines from aconite roots by counter-current chromatography. Journal of Separation Science, 36 (7), 1304-1310. https://doi.org/10.1002/jssc.201201079