School of Chinese Medicine
Inhibitory effect of Astragalus polysaccharides on lipopolysaccharide-induced TNF-a and IL-1ß production in THP-1 cells
Astragalus polysaccharides (APS), one of main bioactive components in Astragalus membranaceus Bunge, has been reported to possess anti-inflammatory activities, but the molecular mechanisms behind this activity are largely unknown. This study aimed to investigate expression of inflammatory cytokines and the MAPK/NF-κB pathway in human THP-1 macrophages induced by lipopolysaccharide (LPS). The results showed that the concentrations of TNF-α and IL-1β released from LPS stimulated THP-1 cells increased significantly compared to control (p < 0.01). After treatment with APS, the TNF-α and IL-1β levels were significantly lower than those in the LPS group (p < 0.05). The mRNA expression of TNF-α and IL-1β were also inhibited. Mechanistic studies indicated that APS strongly suppressed NF-κB activation and down-regulated the phosphorylation of ERK and JNK, which are important signaling pathways involved in the production of TNF-α and IL-1β, demonstrating that APS could suppress the production of TNF-α and IL-1β by LPS stimulated macrophages by inhibiting NF-κB activation and ERK and JNK phosphorylation.
Anti-inflammatory, Astragalus polysaccharides, Macrophage, MAPK, NF-κB
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Link to Publisher's Edition
He, Xiaojuan, Jun Shu, Li Xu, Cheng Lu, and Aiping Lu. "Inhibitory effect of Astragalus polysaccharides on lipopolysaccharide-induced TNF-a and IL-1ß production in THP-1 cells." Molecules 17.3 (2012): 3155-3164.