School of Chinese Medicine
Triptolide downregulates treg cells and the level of IL-10, TGF-β, and VEGF in melanoma-bearing mice
Regulatory T cells play a key role in suppressing tumor immunity. Triptolide, a major active component isolated from the Chinese medicinal herb Tripterygium wilfordii, has been proven to possess multiple antitumor activities. Here, we investigated the effect of triptolide on regulatory T cells and on the level of IL-10, transforming growth factor-β, and vascular endothelial growth factor in tumor-bearing mice. Fifty male C57BL/6 mice were randomly grouped as follows: normal control group, model group with B16-F10 cells implanted, and three treatment groups with cyclophosphamide, triptolide-high dose, triptolide-low dose. The proportion of regulatory T cells in the spleen and axillary lymph nodes was evaluated by flow cytometric analysis. Production of cytokines IL-10, transforming growth factor-β, and vascular endothelial growth factor in serum was measured using enzyme-labeled immunosorbent assay kits. The mRNA levels of Foxp3, IL-10, and transforming growth factor-β in the spleen and vascular endothelial growth factor in tumor tissue were detected by real-time PCR. The results showed that triptolide significantly decreased the proportion of regulatory T cells and lowered the Foxp3 level in the spleen and axillary lymph nodes of tumor-bearing mice. Production of IL-10 and transforming growth factor-β in peripheral blood, and the mRNA level of IL-10 and transforming growth factor-β in the spleen were also decreased. Additionally, triptolide could remarkably inhibit production of vascular endothelial growth factor in tumor-bearing mice. In conclusion, our study demonstrated that triptolide might inhibit tumor growth by inhibiting regulatory T cells and some cytokines such as IL-10 and transforming growth factor-β, as well as production of vascular endothelial growth factor. © Georg Thieme Verlag KG Stuttgart.
Celastraceae, cytokines, melanoma, regulatory T cells, Tripterygium wilfordii, triptolide
Source Publication Title
Georg Thieme Verlag
Link to Publisher's Edition
Liu, Biao, Hongqi Zhang, Jian Li, Cheng Lu, Gao Chen, Ge Zhang, Aiping Lu, and Xiaojuan He. "Triptolide downregulates treg cells and the level of IL-10, TGF-β, and VEGF in melanoma-bearing mice." Planta Medica 79.15 (2013): 1401-1407.