School of Chinese Medicine
In this article, reversal activities of Euphorbia factor L1 (EFL1) against ABCB1-mediated multidrug resistance (MDR) and apoptosis sensitization in K562/ADR cells are reported. EFL1 decreased the IC50 values of anticancer agents in K562/ADR cells over-expressing ABCB1. However, EFL1 did not affect the IC50 values of anticancer agents in sensitive K562 cells. Additionally, EFL1 increased the intracellular accumulation of rhodamine 123 and doxorubicin in K562/ADR cells without affecting their accumulation in K562 cells. Furthermore, EFL1 sensitized the apoptosis triggered by vincristine in K562/ADR cells via mitochondrial pathway, as confirmed by Annexin V-FITC/PI detection and western blot. At the same time, EFL1 did not influence the apoptosis induced by vincristine in K562 cells. Western blot results showed that EFL1 did not affect the phosphorylation level of AKT and ERK in K562 and K562/ADR cells. Finally, EFL1 did not down-regulate protein expression of ABCB1.
ABCB1, Apoptosis, Euphorbia factor L1, Multidrug resistance
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
This work was supported by the grants from Guangdong Science and Technology Department & Guangdong Academy of Traditional Chinese Medicine (2012A032500021), research Project of Guangzhou Education Bureau (10A167) and funding from Guangzhou Science and Technology Program (2013J4100033).
Link to Publisher's Edition
Zhang, J., Lin, M., Yi, T., Tang, Y., Fan, L., He, X., Zhao, Z., & Chen, H. (2013). Apoptosis sensitization by euphorbia factor L1 in ABCB1-mediated multidrug resistant K562/ADR cells. Molecules, 18 (10), 12793-12808. https://doi.org/10.3390/molecules181012793