School of Chinese Medicine
A new lignan, vitexkarinol (1), as well as a known lignan, neopaulownin (2), a known chalcone, 3-(4-hydroxyphenyl)-1-(2,4,6-trimethoxyphenyl)-2-propen- 1-one (3), two known dehydroflavones, tsugafolin (4) and alpinetin (5), two known dipeptides, aurantiamide and aurantiamide acetate, a known sesquiterpene, vemopolyanthofuran, and five known carotenoid metabolites, vomifoliol, dihydrovomifoliol, dehydrovomifoliol, loliolide, and isololiolide, were isolated from the leaves and twigs of Vitex leptobotrys through bioassay-guided fractionation. The chalcone (3) was found to inhibit HIV-1 replication by 77% at 15.9 μM, and the two dehydroflavones (4 and 5) showed weak anti-HIV activity with IC50 values of 118 and 130 μM, respectively, while being devoid of cytotoxicity at 150 μM. A chlorophyll-enriched fraction of V. leptobotrys, containing pheophorbide a, was found to inhibit the replication of HIV-1 by 80% at a concentration of 10 μg/mL. Compounds 1 and 3 were further selected to be evaluated against 21 viral targets available at NIAID (National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA).
Source Publication Title
Journal of Natural Products
American Chemical Society
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Natural Products, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/np400779v
The work described in this paper was supported by NIH Grants 3U01TW001015-10S1 and 2U01TW001015-11A1 (administered by the Fogarty International Center as part of an International Cooperative Biodiversity Groups program, through funds from NIH, NSF, and Foreign Agricultural Service of the USDA), the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. HKBU 262912), and Faculty Research Grant, Hong Kong Baptist University (FRG2/11-12/134).
Link to Publisher's Edition
Pan, Wenhui, Kanglun Liu, Yifu Guan, Ghee Teng Tan, Nguyen Van Hung, Nguyen Manh Cuong, D. Doel Soejarto, John M. Pezzuto, Harry H. S. Fong, and Hongjie Zhang. "Bioactive compounds from Vitex leptobotrys." Journal of Natural Products 77.3 (2014): 663-667.