School of Chinese Medicine
Rabdosia japonica var. glaucocalyx (Maxim.) Hara, belonging to the Labiatae family, is widely used as an anti-inflammatory and antitumor drug for the treatment of different inflammations and cancers. Aim of the Study. To investigate therapeutic effects and possible mechanism of the flavonoids fraction of Rabdosia japonica var. glaucocalyx (Maxim.) Hara (RJFs) in acute lung injury (ALI) mice induced by lipopolysaccharide (LPS). Materials and Methods. Mice were orally administrated with RJFs (6.4, 12.8, and 25.6 mg/kg) per day for 7 days, consecutively, before LPS challenge. Lung specimens and the bronchoalveolar lavage fluid (BALF) were isolated for histopathological examinations and biochemical analysis. The level of complement 3 (C3) in serum was quantified by a sandwich ELISA kit. Results. RJFs significantly attenuated LPS-induced ALI via reducing productions of the level of inflammatory mediators (TNF-α, IL-6, and IL-1β), and significantly reduced complement deposition with decreasing the level of C3 in serum, which was exhibited together with the lowered myeloperoxidase (MPO) activity and nitric oxide (NO) and protein concentration in BALF. Conclusions. RJFs significantly attenuate LPS-induced ALI via reducing productions of proinflammatory mediators, decreasing the level of complement, and reducing radicals.
Source Publication Title
Evidence-Based Complementary and Alternative Medicine
Article ID 894515
Hindawi Publishing Corporation
Copyright © 2014 Chun-jun Chu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The authors acknowledge Mr. Alan Ho from the School of Chinese Medicine, Hong Kong Baptist University, for his technical supports. This work was financially supported by the Natural Science Fund for Colleges and Universities in Jiangsu Province (no. 10KJB350004), Suzhou Application Foundation Research Project (no. SYS201206), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Link to Publisher's Edition
Chu, Chun-Jun, Nai-Yu Xu, Xian-Lun Li, Long Xia, Jian Zhang, Zhi-Tao Liang, Zhong-Zhen Zhao, and Dao-Feng Chen. "Rabdosia japonica var. glaucocalyx flavonoids fraction attenuates lipopolysaccharide-induced acute lung injury in mice." Evidence-Based Complementary and Alternative Medicine 2014 (2014): Article ID 894515.