Document Type
Journal Article
Department/Unit
School of Chinese Medicine
Title
Magnolol inhibits colonic motility through down-regulation of voltage-sensitive L-type Ca2+ channels of colonic smooth muscle cells in rats
Language
English
Abstract
This study aimed to investigate the effect of magnolol (5,5′-diallyl- 2,2′-biphenyldiol) on contraction in distal colonic segments of rats and the underlying mechanisms. Colonic segments were mounted in organ baths for isometric force measurement. Whole-cell voltage-sensitive L-type Ca2+ currents were recorded on isolated single colonic smooth muscle cells using patch-clamp technique. The spontaneous contractions and acetylcholine (ACh)- and Bay K 8644-induced contractions were inhibited by magnolol (3-100 μM). In the presence of Bay K8644 (100 nM), magnolol (10-100 μM) inhibited the contraction induced by 10 μM ACh. By contrast, tetrodotoxin (100 nM) and Nώ-nitro-l-arginine methyl ester (l-NAME 100 μM) did not change the inhibitory effect of magnolol (10 μM). In addition, magnolol (3-100 μM) inhibited the L-type Ca2+ currents. The present results suggest that magnolol inhibits colonic smooth muscle contraction through downregulating L-type Ca2+ channel activity. © 2013 Elsevier GmbH.
Keywords
Colonic motility, L-type Ca2+ channels, Magnolia officinalis, Magnolol, Smooth muscle cells
Publication Date
2013
Source Publication Title
Phytomedicine
Volume
20
Issue
4
Start Page
1272
End Page
1279
Publisher
Elsevier
DOI
10.1016/j.phymed.2013.07.008
Link to Publisher's Edition
http://dx.doi.org/10.1016/j.phymed.2013.07.008
ISSN (print)
09447113
ISSN (electronic)
1618095X
APA Citation
Zhang, M., Zang, K., Luo, J., Leung, F., Huang, Y., Lin, C., Yang, Z., Lu, A., Tang, X., Xu, H., Sung, J., & Bian, Z. (2013). Magnolol inhibits colonic motility through down-regulation of voltage-sensitive L-type Ca2+ channels of colonic smooth muscle cells in rats. Phytomedicine, 20 (4), 1272-1279. https://doi.org/10.1016/j.phymed.2013.07.008