School of Chinese Medicine
Astragalus polysaccharides (APS) possess multiple immunomodulatory activities. Due to its high molecular weight, orally administration of APS is not easily absorbed into the blood stream, and how APS exerts its capacity in vivo is still not well elucidated. We assume that enteric mucosal immune response might trigger the immune regulation of APS, and our previous studies demonstrated that APS had regulatory activity on intraepithelial lymphocytes (IELs). Therefore, this study aimed to investigate the functions of APS on intestinal intraepithelial γδT cells, a major subset in IELs and an essential component of maintaining homeostasis and immune regulation in enteric mucosa. Results showed that APS could promote proliferation and function of intestinal intraepithelial γδT cells in vitro, the IFN-γ, FasL and GrB mRNA levels in γδT cells were all significantly increased. Moreover, APS also improved the activity of intestinal intraepithelial γδT cells in vivo, as cytokines production and cytotoxicity of γδT cells were all remarkably improved in tumor-bearing mice treated with APS. In addition, the levels of TNF-α and IFN-γ were significantly increased, whereas the levels of IL-10 and TGF-β were significantly decreased in tumor-bearing mice treated with APS. In conclusion, this study demonstrated that APS could improve proliferation and function of intestinal intraepithelial γδT cells, which might an important pathway for immunomodulation of APS in cancer therapy.
Astragalus polysaccharides, Enteric mucosa, Tumor, γδT cells
Source Publication Title
© 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license.
This research is supported in part by the projects from National Science Foundation of China (No. 81001676) and by E-institutes of Shanghai Municipal Education Commission (No. E03008).
Link to Publisher's Edition
Sun, Shuyu, Kang Zheng, Hongyan Zhao, Cheng Lu, Biao Liu, Changyuan Yu, Ge Zhang, Zhaoxiang Bian, Aiping Lu, and Xiaojuan He. "Regulatory effect of astragalus polysaccharides on intestinal intraepithelial γδt cells of tumor bearing mice." Molecules 19.9 (2014): 15224-15236.