School of Chinese Medicine
Isolation and structural characterization of an immunostimulating polysaccharide from fuzi, Aconitum carmichaeli
A water-soluble polysaccharide named as FPS-1 was isolated from fuzi, the root of Aconitum carmichaeli Debx. by hot-water extraction, anion-exchange and gel-permeation chromatography and tested for its pharmacological activities. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, methylation analysis and GLC-MS. Based on the data obtained, FPS-1 was determined to be an α-(1→6)-d-glucan, with a weight-average molecular weight of about 14,000 Da. The glucan is highly branched with a single glucose at the C-3 position every four residues, on average, along the main chain. In immunopharmacological studies, FPS-1 showed potent stimulating effects on murine lymphocyte proliferation induced by concanavalin A or lipopolysaccharide both in vitro and in vivo as well as on splenocyte antibody production. © 2005 Elsevier Ltd. All rights reserved.
α-D-Glucan, Aconitum carmichaeli, Fuzi, Lymphocyte proliferation, Ranunculaceae, Structure
Source Publication Title
Link to Publisher's Edition
Zhao, C., Li, M., Luo, Y., & Wu, W. (2006). Isolation and structural characterization of an immunostimulating polysaccharide from fuzi, Aconitum carmichaeli. Carbohydrate Research, 341 (4), 485-491. https://doi.org/10.1016/j.carres.2005.11.032